Table 1 Primers used in the experiment.
From: NBR1-p62-Nrf2 mediates the anti-pulmonary fibrosis effects of protodioscin
Gene | Forward [5'-3'] | Reverse [5'-3'] |
|---|---|---|
Mouse collagen I | GAGCGGAGAGTACTGGATCG | GCTTCTTTTCCTTGGGGTTC |
Mouse α-SMA | TGGCTATTCAGGCTGTGCTGTC | CAATCTCACGCTCGGCAGTAGT |
Mouse β-Actin | GTGCTATGTTGCTCTAGACTTCG | ATGCCACAGGATTCCATACC |
Mouse FN1 | AGTGGCTGAAGTCGCAAGGAAAC | TAAGTCTGGGTCACGGCTGTCTC |
Mouse CTGF | CACCGCACAGAACCACCACTC | AATGGCAGGCACAGGTCTTGATG |
Rat β-Actin | TGTCACCAACTGGGACGATA | GGGGTGTTGAAGGTCTCAAA |
Rat α-SMA | GCGTGGCTATTCCTTCGTGACTAC | CATCAGGCAGTTCGTAGCTCTTCTC |
Rat collagen I | TGTTGGTCCTGCTGGCAAGAATG | GTCACCTTGTTCGCCTGTCTCAC |
Rat FN1 | AGGCACAAGGTCCGAGAAGAGG | CATGAGTCATCCGTAGGCTGGTTC |
Rat CTGF | CACCGCACAGAACCACCACAC | AATGGCAGGCACAGGTCTTGATG |
Human β-Actin | CCTGGCACCCAGCACAAT | GGGCCGGACTCGTCATAC |
Human α-SMA | TCCGGAGCGAAATACTCTG | CCCGGCTTCATCGTATTCCT |
Human collagen I | CCACCAATCACCTGCGTACA | CACGTCATCGCACAACACCT |
Human FN1 | GGCGACAGGACGGACATCTTTG | GGCACAAGGCACCATTGGAATTTC |
Human CTGF | CGGGAAATGCTGCGAGGAGTG | GGTCTGGGCCAAACGTGTCTTC |
Mouse Nrf2 | AGTCCAGAAGCCAAACTGACAGAAG | GGAGAGGATGCTGCTGAAGGAATC |
Mouse HO-1 | ACCGCCTTCCTGCTCAACATTG | CTCTGACGAAGTGACGCCATCTG |
Mouse NQO1 | GCGAGAAGAGCCCTGATTGTACTG | AGCCTCTACAGCAGCCTCCTTC |
Mouse p62 | AGGAGGAGACGATGACTGGACAC | TTGGTCTGTAGGAGCCTGGTGAG |
Mouse NBR1 | AACAGGACTCGCAAACAGCAGAC | GTACGCAGAGGCAGCAACAGAC |
Mouse PRKCI | TGACTACGGCATGTGTAAGGAAGG | CCAGTCAACGCTGAAGCCATAATC |
Mouse WDFY3 | TCACCAGCAGCCGATGTAAGC | GAAGAGACCACCGTTCCGCTAG |
Mouse CYLD | TTCCAGGAGTTGTACGCTTCAGAG | AACCTTGACCACGACCTTCTTCC |
Mouse TRAF6 | ACTGCCCAACAGCTCCAATCC | CAAGTGTCGTGCCAAGTGATTCC |