Fig. 4

Transcriptome analysis reveals BZBS improving inflammatory and SASP. The number of differentially expressed genes (DEGs) in RNA-seq analysis of young vs. aged and BZBS vs. aged in perirenal fat (A, B) and liver (C, D) shown in volcano plots. Intersection of DEGs in Venn diagrams for young vs. aged (Y vs A) and BZBS vs. aged (B vs A) in perirenal fat (E) and liver (F). KEGG pathway analysis of intersection DEGs in perirenal fat (G) and liver (H). Gene Set Enrichment Analysis (GSEA) of TNF signaling pathway (I), IL-17 signaling pathway (J), and SASP (K) in perirenal fat and liver RNA-seq. The mRNA expression of inflammation-related genes by qRT-PCR (L)