Fig. 3

OP inhibits autophagy in tBHP-induced H9C2 cells. A The LC3B and P62 proteins expression in tBHP treatment H9C2 cells for different time points of 30, 60, 120, 240 min. B The effect of OP on the protein expressions of LC3B and P62 in tBHP-induced H9C2 cells as indicated. C H9c2 cells were pretreated with or without CQ (5 μM) for 2 h and then treated or untreated with OP for other 2 h, after which acted with tBHP (150 μM) for 4 h, the cell viability was detected by MTT assay. D The effect of OP or CQ on the protein expressions of LC3B and P62 in tBHP-induced H9C2 cells as indicated. E The level of P62 was detected by immunofluorescence. F The fluorescence levels of LC3B in H9C2 cells infected with stubRFP-sensGFP-LC3 lentivirus. All data are expressed as mean ± SEM, n = 3. ^###P < 0.005 vs Control; ^**P < 0.01, ^***P < 0.005 vs tBHP